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Several transgenic zebrafish lines for liver development studies had been obtained in the first decade of this century, but not any transgenic GFP zebrafish lines that mark the through liver development and organogenesis were reported. In this study, we analyzed expression pattern of endogenous  Apo-14  in zebrafish embryogenesis by whole-mount  in situ  hybridization, and revealed its expression in liver primordium and in the following liver development. Subsequently, we isolated zebrafish  Apo-14  promoter of 1763 bp 5′-flanking sequence, and developed an  Apo-14  promoter-driven transgenic zebrafish  Tg ( Apo14: GFP ). And, maternal expression and post-fertilization translocation of  Apo-14  promoter-driven GFP were observed in the transgenic zebrafish line. Moreover, we traced onset expression of  Apo-14  promoter-driven GFP and developmental behavior of the expressed cells in early heterozygous embryos by out-crossing the  Tg ( Apo14: GFP ) male to the wild type female. Significantly, the  Apo-14  promoter-driven GFP is initially expressed around YSL beneath the embryo body at 10 hpf when the embryos develop to tail bud prominence. In about 14-somite embryos at 16–17 hpf, a typical “salt-and-pepper” expression pattern is clearly observed in YSL around the yolk sac. Then, a green fluorescence dot begins to appear between the notochord and the yolk sac adjacent to otic vesicle at about 20 hpf, which is later demonstrated to be liver primordium that gives rise to liver. Furthermore, we investigated dynamic progression of liver organogenesis in the  Tg ( Apo14: GFP ) zebrafish, because the  Apo-14  promoter-driven GFP is sustainably expressed from hepatoblasts and liver progenitor cells in liver primordium to hepatocytes in the larval and adult liver. Additionally, we observed similar morphology between the liver progenitor cells and the GFP-positive nuclei on the YSL, suggesting that they might originate from the same progenitor cells in early embryos. Overall, the current study provides a transgenic zebrafish line that marks the through liver organogenesis.

An Apo-14 Promoter-Driven Transgenic Zebrafish That Marks Liver Organogenesis