English (United Kingdom)

https://curated-unify.zendy.io/wp-json/zendy-region/v1/featured_content/oa?rat=en

https://curated-unify.zendy.io/wp-json/zendy-region/v1/highlighted_journal/

Global: Zendy Plus annual

Presents the access of premium content as premium feature

Premium Content

Presents the keyphrase highlighting as premium feature

Keyphrase Highlighting

Presents the summarisation as premium feature

Summarisation

Insights

Presents the pdf analysis as premium feature

PDF Analysis

Presents the zaia usage as premium feature

ZAIA

Global: Zendy Tools annual

Global: Zendy Free Plan

Global: Zendy Tools monthly

Global: Zendy Plus monthly

Background  The conserved SNF1/AMPK/SnRK1 complexes are global regulators of metabolic responses in eukaryotes and play a key role in the control of energy balance. Although α-type subunits of the SnRK1 complex have been characterized in several plant species, the biological function of β-type and γ-type subunits remains largely unknown. Here, we characterized  AtPV42a  and  AtPV42b , the two homologous genes in  Arabidopsis , which encode cystathionine-β-synthase (CBS) domain-containing proteins that belong to the PV42 class of γ-type subunits of the plant SnRK1 complexes.    Methodology/Principal Findings  Real-time polymerase chain reaction was performed to examine the expression of  AtPV42a  and  AtPV42b  in various tissues. Transgenic plants that expressed artificial microRNAs targeting these two genes were created. Reproductive organ development and fertilization in these plants were examined by various approaches, including histological analysis, scanning electron microscopy, transmission electron microscopy, and phenotypic analyses of reciprocal crosses between wild-type and transgenic plants. We found that  AtPV42a  and  AtPV42b  were expressed in various tissues during different developmental stages. Transgenic plants where  AtPV42a  and  AtPV42b  were simultaneously silenced developed shorter siliques and reduced seed sets. Such low fertility phenotype resulted from deregulation of late stamen development and impairment of pollen tube attraction conferred by the female gametophyte.    Conclusions  Our results demonstrate that  AtPV42a  and  AtPV42b  play redundant roles in regulating male gametogenesis and pollen tube guidance, indicating that the  Arabidopsis  SnRK1 complexes might be involved in the control of reproductive development.

AtPV42a and AtPV42b Redundantly Regulate Reproductive Development in Arabidopsis thaliana