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Use of ITS2 Region as the Universal DNA Barcode for Plants and Animals
Author(s) -
Hui Yao,
Jingyuan Song,
Chang Liu,
Kun Luo,
Jianping Han,
Ying Li,
Xiaohui Pang,
HongXi Xu,
Yingjie Zhu,
PeiGen Xiao,
Shilin Chen
Publication year - 2010
Publication title -
plos one
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.99
H-Index - 332
ISSN - 1932-6203
DOI - 10.1371/journal.pone.0013102
Subject(s) - biology , genbank , internal transcribed spacer , phylogenetic tree , dna barcoding , evolutionary biology , ribosomal dna , barcode , locus (genetics) , genetics , gc content , phylogenetics , ribosomal rna , genome , gene , computer science , operating system
The internal transcribed spacer 2 (ITS2) region of nuclear ribosomal DNA is regarded as one of the candidate DNA barcodes because it possesses a number of valuable characteristics, such as the availability of conserved regions for designing universal primers, the ease of its amplification, and sufficient variability to distinguish even closely related species. However, a general analysis of its ability to discriminate species in a comprehensive sample set is lacking.

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