A Full-Genomic Sequence-Verified Protein-Coding Gene Collection for Francisella tularensis | Zendy

Tal V. Murthy | Zendy; Andreas Rolfs | Zendy; Yanhui Hu | Zendy; Zhenwei Shi | Zendy; Jacob Raphael | Zendy; Donna Moreira | Zendy; Fontina Kelley | Zendy; Seamus McCarron | Zendy; Daniel A. Jepson | Zendy; Elena Taycher | Zendy; Dongmei Zuo | Zendy; Stephanie E. Mohr | Zendy; Mauricio Fernández | Zendy; Leonardo Brizuela | Zendy; Joshua LaBaer | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

A Full-Genomic Sequence-Verified Protein-Coding Gene Collection for Francisella tularensis

Author(s) -

Tal V. Murthy,

Andreas Rolfs,

Yanhui Hu,

Zhenwei Shi,

Jacob Raphael,

Donna Moreira,

Fontina Kelley,

Seamus McCarron,

Daniel A. Jepson,

Elena Taycher,

Dongmei Zuo,

Stephanie E. Mohr,

Mauricio Fernández,

Leonardo Brizuela,

Joshua LaBaer

Publication year - 2007

Publication title -

plos one

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.99

H-Index - 332

ISSN - 1932-6203

DOI - 10.1371/journal.pone.0000577

Subject(s) - francisella tularensis , clone (java method) , biology , plasmid , gene , computational biology , coding region , genome , genetics , bioinformatics , virulence

The rapid development of new technologies for the high throughput (HT) study of proteins has increased the demand for comprehensive plasmid clone resources that support protein expression. These clones must be full-length, sequence-verified and in a flexible format. The generation of these resources requires automated pipelines supported by software management systems. Although the availability of clone resources is growing, current collections are either not complete or not fully sequence-verified. We report an automated pipeline, supported by several software applications that enabled the construction of the first comprehensive sequence-verified plasmid clone resource for more than 96% of protein coding sequences of the genome of F. tularensis , a highly virulent human pathogen and the causative agent of tularemia. This clone resource was applied to a HT protein purification pipeline successfully producing recombinant proteins for 72% of the genes. These methods and resources represent significant technological steps towards exploiting the genomic information of F. tularensis in discovery applications.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research