DNA Display I. Sequence-Encoded Routing of DNA Populations | Zendy

David R Halpin | Zendy; Pehr B. Harbury | Zendy

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DNA Display I. Sequence-Encoded Routing of DNA Populations

Author(s) -

David R Halpin,

Pehr B. Harbury

Publication year - 2004

Publication title -

plos biology

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 4.127

H-Index - 271

eISSN - 1545-7885

pISSN - 1544-9173

DOI - 10.1371/journal.pbio.0020173

Subject(s) - biology , oligonucleotide , modular design , computational biology , dna , dna sequencing , routing (electronic design automation) , sequence (biology) , genetics , computer science , computer network , programming language

Recently reported technologies for DNA-directed organic synthesis and for DNA computing rely on routing DNA populations through complex networks. The reduction of these ideas to practice has been limited by a lack of practical experimental tools. Here we describe a modular design for DNA routing genes, and routing machinery made from oligonucleotides and commercially available chromatography resins. The routing machinery partitions nanomole quantities of DNA into physically distinct subpools based on sequence. Partitioning steps can be iterated indefinitely, with worst-case yields of 85% per step. These techniques facilitate DNA-programmed chemical synthesis, and thus enable a materials biology that could revolutionize drug discovery.

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