Microdissection-derived Murine Mcb Probes from Somatic Cell Hybrids | Zendy

Vladimir A. Trifonov | Zendy; Constanze Karst | Zendy; Uwe Claussen | Zendy; Kristin Mrasek | Zendy; Susanne Michel | Zendy; Philip Avner | Zendy; Thomas Liehr | Zendy

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Microdissection-derived Murine Mcb Probes from Somatic Cell Hybrids

Author(s) -

Vladimir A. Trifonov,

Constanze Karst,

Uwe Claussen,

Kristin Mrasek,

Susanne Michel,

Philip Avner,

Thomas Liehr

Publication year - 2005

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1369/jhc.4b6598.2005

Subject(s) - microdissection , fluorescence in situ hybridization , somatic cell , biology , microbiology and biotechnology , genetics , fish <actinopterygii> , chromosome , computational biology , gene , fishery

The multicolor-banding (mcb) technique is a fluorescence in situ hybridization (FISH)-banding approach, which is based on region-specific microdissection libraries producing changing fluorescence intensity ratios along the chromosomes. The latter are used to assign different pseudocolors to specific chromosomal regions. Here we present the first three available mcb-probe sets for the Mus musculus chromosomes 3, 6, and 18. In the present work, the creation of the microdissection libraries was done for the first time on mouse/human somatic cell hybrids. During creation of the mcb-probes, the latter enabled an unambiguous identification of the, otherwise in GTG-banding, hardly distinguishable murine chromosomes.

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