The Usefulness of Calyculin A for Cytogenetic Prenatal Diagnosis | Zendy

Malgorzata I. Srebniak | Zendy; Gizela G. Trapp | Zendy; Angelika Wawrzkiewicz | Zendy; Wojciech Kaźmierczak | Zendy; Andrzej Wiczkowski | Zendy

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The Usefulness of Calyculin A for Cytogenetic Prenatal Diagnosis

Author(s) -

Malgorzata I. Srebniak,

Gizela G. Trapp,

Angelika Wawrzkiewicz,

Wojciech Kaźmierczak,

Andrzej Wiczkowski

Publication year - 2005

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.971

H-Index - 124

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1369/jhc.4b6392.2005

Subject(s) - metaphase , premature chromosome condensation , serine , phosphatase , chromosome , cell cycle , biology , prenatal diagnosis , genetics , microbiology and biotechnology , threonine , biochemistry , cell , fetus , phosphorylation , gene , pregnancy

An increased number of chromosome plates can be obtained by use of calyculin A (CLA). CLA is an inhibitor of protein phosphatases (type 1 and type 2A serine/threonine). Inactivation of these phosphatases leads to premature chromosome condensation (PCC) in all phases of the cell cycle; thus, it is possible to investigate both metaphase and G 2 -PCC chromosomes. Amniotic fluid (AF) cultures were treated with calyculin A (CLA). GTG banding was obtained. Using this method it is possible to investigate all cell cycle phases, GTG banding, chromosomal breaks, and rates of PCD on the same preparation. Analyses of AF cultures treated with CLA allow complex studies on fetal genetic material. This work presents potential usefulness of CLA for cytogenetic prenatal diagnosis.

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