Cell and Tissue Display | Zendy

Nicholas Theodosakis | Zendy; Goran Micevic | Zendy; Marcus Bosenberg | Zendy; Nemanja Rodić | Zendy

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Cell and Tissue Display

Author(s) -

Nicholas Theodosakis,

Goran Micevic,

Marcus Bosenberg,

Nemanja Rodić

Publication year - 2016

Publication title -

journal of histochemistry and cytochemistry

Language(s) - English

Resource type - Journals

eISSN - 1551-5044

pISSN - 0022-1554

DOI - 10.1369/0022155416651065

Subject(s) - ctd , agarose , compartment (ship) , block (permutation group theory) , biomedical engineering , computer science , pathology , biology , medicine , geology , microbiology and biotechnology , oceanography , geometry , mathematics

We developed a method, termed Cell and Tissue Display (CTD), for embedding 16 or more different tissue samples in multi-compartment agarose blocks. The CTD-generated blocks allow uniform multiplexing of cell lines and small tissue fragments within a single histologic block. The distribution of individual cells within the CTD blocks is improved, likely because the individual agarose compartments are small and uniform. The composition of each CTD block can be customized based on intended use. Some potential uses of CTD histologic blocks include improved sectioning of small tissue fragments, such as needle biopsy specimens or punch biopsies; multiplexing of tissue fragments within a single block; and the generation of control slides for laboratory proficiency testing.

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