Raman imaging of floating cells
Author(s) -
Caitriona M. Creely,
Giovanni Volpe,
Gajendra Singh,
Marta Soler,
Dmitri Petrov
Publication year - 2005
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/opex.13.006105
Subject(s) - raman spectroscopy , optical tweezers , optics , suspension (topology) , holography , materials science , raman scattering , physics , mathematics , homotopy , pure mathematics
Raman imaging can yield spatially resolved biochemical information from living cells. To date there have been no Raman images published of cells in suspension because of the problem of immobilizing them suitably to acquire space-resolved spectra. In this paper in order to overcome this problem the use of holographic optical tweezers is proposed and implemented, and data is shown for spatially resolved Raman spectroscopy of a live cell in suspension.
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