Spiral phase contrast imaging in microscopy | Zendy

Severin Fürhapter | Zendy; Alexander Jesacher | Zendy; Stefan Bernet | Zendy; Monika RitschMarte | Zendy

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Spiral phase contrast imaging in microscopy

Author(s) -

Severin Fürhapter,

Alexander Jesacher,

Stefan Bernet,

Monika RitschMarte

Publication year - 2005

Publication title -

optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.394

H-Index - 271

ISSN - 1094-4087

DOI - 10.1364/opex.13.000689

Subject(s) - optics , holography , spatial light modulator , phase (matter) , optical vortex , digital holographic microscopy , fourier transform , ptychography , spatial filter , diffraction , microscopy , spatial frequency , spiral (railway) , image plane , physics , materials science , computer science , mathematical analysis , mathematics , quantum mechanics , beam (structure) , artificial intelligence , image (mathematics)

We demonstrate an optical method for edge contrast enhancement in light microscopy. The method is based on holographic Fourier plane filtering of the microscopic image with a spiral phase element (also called vortex phase or helical phase filter) displayed as an off-axis hologram at a computer controlled high resolution spatial light modulator (SLM) in the optical imaging pathway. The phase hologram imprints a helical phase term of the form exp(i phi) on the diffracted light field in its Fourier plane. In the image plane, this results in a strong and isotropic edge contrast enhancement for both amplitude and phase objects.

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