Two-photon fluorescence microscope with a hollow-core photonic crystal fiber | Zendy

Shih-Peng Tai | Zendy; MingChe Chan | Zendy; Tsung-Han Tsai | Zendy; Shi-Hao Guol | Zendy; Li-Jin Chen | Zendy; ChiKuang Sun | Zendy

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Two-photon fluorescence microscope with a hollow-core photonic crystal fiber

Author(s) -

Shih-Peng Tai,

MingChe Chan,

Tsung-Han Tsai,

Shi-Hao Guol,

Li-Jin Chen,

ChiKuang Sun

Publication year - 2004

Publication title -

optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.394

H-Index - 271

ISSN - 1094-4087

DOI - 10.1364/opex.12.006122

Subject(s) - optics , materials science , photonic crystal fiber , femtosecond , dispersion (optics) , self phase modulation , microscopy , fiber , microscope , zero dispersion wavelength , optical fiber , fluorescence , wavelength , dispersion shifted fiber , optoelectronics , nonlinear optics , laser , fiber optic sensor , physics , composite material

Self-phase-modulation and group velocity dispersion of near IR femtosecond pulses in fibers restrict their use in two-photon fluorescence microscopy (TPFM). Here we demonstrate a hollow-core photonic crystal fiber based two-photon fluorescence microscope with low nonlinearity and dispersion effects. We use this fiber-based TPFM system to take two-photon fluorescence (chlorophyll) images of mesophyll tissue in the leaf of Rhaphidophora aurea. With less than 2mW average power exposure on the leaf at 755nm, the near zero-dispersion wavelength, chloroplasts distribution inside the mesophyll cells can be identified with a sub-micron spatial resolution. The acquired image quality is comparable to that acquired by the conventional fiber-free TPFM system, due to the negligible temporal pulse broadening effect.

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