Open AccessAssessing patterns for compressive fluorescence lifetime imaging
Author(s) -
Marien Ochoa,
Qi Pian,
Ru Yao,
Nicolas Ducros,
Xavier Intes
Publication year - 2018
Publication title -
optics letters/optics index
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.524
H-Index - 272
eISSN - 1071-2763
pISSN - 0146-9592
DOI - 10.1364/ol.43.004370
Subject(s) - hyperspectral imaging , optics , compressed sensing , hadamard transform , materials science , fluorescence , imaging phantom , fluorescence lifetime imaging microscopy , digital micromirror device , förster resonance energy transfer , computer science , physics , artificial intelligence , quantum mechanics
A novel hyperspectral single pixel system was used to compare different compressive basis patterns for intensity imaging, lifetime imaging, and FRET quantification. Six popular basis patterns were compared experimentally in a phantom containing two fluorescent dyes. The basis patterns that performed best for lifetime quantification were used to measure FRET occurrence in well-plate samples with varying acceptor-donor ratios. The ABS-WP approach using Haar patterns and the compressive sensing approach with Hadamard Ranked patterns displayed the best overall performances at a 50% compression ratio.