Particle tracking by repetitive phase-shift interferometric super resolution microscopy | Zendy

Itay Gdor | Zendy; X. Wang | Zendy; Matthew K. Daddysman | Zendy; Yuval Yifat | Zendy; Rosemarie Wilton | Zendy; Mark Hereld | Zendy; MarieFrançoise NoirotGros | Zendy; Norbert F. Scherer | Zendy

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Particle tracking by repetitive phase-shift interferometric super resolution microscopy

Author(s) -

Itay Gdor,

X. Wang,

Matthew K. Daddysman,

Yuval Yifat,

Rosemarie Wilton,

Mark Hereld,

MarieFrançoise NoirotGros,

Norbert F. Scherer

Publication year - 2018

Publication title -

optics letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.524

H-Index - 272

eISSN - 1071-2763

pISSN - 0146-9592

DOI - 10.1364/ol.43.002819

Subject(s) - optics , interferometry , microscopy , tracking (education) , super resolution microscopy , phase (matter) , resolution (logic) , particle (ecology) , interference microscopy , materials science , physics , computer science , scanning confocal electron microscopy , artificial intelligence , pedagogy , oceanography , geology , quantum mechanics , psychology

Accurate and rapid particle tracking is essential for addressing many research problems in single molecule and cellular biophysics and colloidal soft condensed matter physics. We developed a novel three-dimensional interferometric fluorescent particle tracking approach that does not require any sample scanning. By periodically shifting the interferometer phase, the information stored in the interference pattern of the emitted light allows localizing particles positions with nanometer resolution. This tracking protocol was demonstrated by measuring a known trajectory of a fluorescent bead with sub-5 nm axial localization error at 5 Hz. The interferometric microscopy was used to track the RecA protein in Bacillus subtilis bacteria to demonstrate its compatibility with biological systems.

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