Open AccessHigh speed optical holography of retinal blood flow
Author(s) -
Mathilde Pellizzari,
Manuel Simonutti,
Julie Degardin,
José-Alain Sahel,
Mathias Fink,
Michel Paques,
Michael Atlan
Publication year - 2016
Publication title -
hal (le centre pour la communication scientifique directe)
Language(s) - English
DOI - 10.1364/ol.41.003503.v006
Subject(s) - holography , retinal , blood flow , optics , flow (mathematics) , physics , computer science , materials science , ophthalmology , medicine , mechanics , cardiology
International audienceWe performed non-invasive video imaging of retinal blood flow in a pigmented rat by holographic interferometry of near-infrared laser light backscattered by retinal tissue, beating against an off-axis reference beam sampled at a frame rate of 39 kHz with a high throughput camera. Local Doppler contrasts emerged from the envelopes of short-time Fourier transforms and the phase of autocorrelation functions of holograms rendered by Fresnel transformation. This approach permitted imaging of blood flow in large retinal vessels (30 microns diameter) over 400 by 400 pixels with a spatial resolution of 8 microns and a temporal resolution of 6.5 ms