Three-dimensional imaging with reflection synthetic confocal microscopy
Author(s) -
Md Rasedujjaman,
Kévin Affannoukoué,
Nicolas GarciaSeyda,
Philippe Robert,
Hugues Giovannini,
Patrick C. Chaumet,
Olivier Théodoly,
MariePierre Valignat,
Kamal Belkebir,
Anne Sentenac,
Guillaume Maire
Publication year - 2020
Publication title -
optics letters
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.524
H-Index - 272
eISSN - 1071-2763
pISSN - 0146-9592
DOI - 10.1364/ol.397364
Subject(s) - optical coherence tomography , microscopy , optics , confocal microscopy , microscope , confocal , tomographic reconstruction , reflection (computer programming) , tomography , resolution (logic) , biological imaging , materials science , computer science , artificial intelligence , physics , fluorescence , programming language
Biomedical imaging lacks label-free microscopy techniques able to reconstruct the contour of biological cells in solution, in 3D and with high resolution, as required for the fast diagnosis of numerous diseases. Inspired by computational optical coherence tomography techniques, we present a tomographic diffractive microscope in reflection geometry used as a synthetic confocal microscope, compatible with this goal and validated with the 3D reconstruction of a human effector T lymphocyte.
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