Quantitative phase microscopy of biological samples using a portable interferometer | Zendy

Natan T. Shaked | Zendy

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Quantitative phase microscopy of biological samples using a portable interferometer

Author(s) -

Natan T. Shaked

Publication year - 2012

Publication title -

optics letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.524

H-Index - 272

eISSN - 1071-2763

pISSN - 0146-9592

DOI - 10.1364/ol.37.002016

Subject(s) - interferometry , optics , interference microscopy , microscopy , phase (matter) , optical path length , microscope , materials science , spatial frequency , coherence (philosophical gambling strategy) , optical coherence tomography , optical path , optical microscope , phase imaging , physics , scanning electron microscope , quantum mechanics

This Letter presents the τ interferometer, a portable and inexpensive device for obtaining spatial interferograms of microscopic biological samples without the strict stability and the highly coherent illumination that are usually required for interferometric microscopy setups. The device is built using off-the-shelf optical elements and can easily operate with low-coherence illumination, while being positioned in the output of a conventional inverted microscope. The interferograms are processed into the quantitative amplitude and phase profiles of the sample. Based on the phase profile, the optical-path-delay profile is obtained with temporal stability of 0.18 nm and spatial stability of 0.42 nm. Further experimental demonstration of using the τ interferometer for imaging the quantitative thickness profile of a live red blood cell is provided.

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