Total-internal-reflection fluorescence microscopy with W-shaped axicon mirrors | Zendy

Ming Lei | Zendy; Andreas Zumbusch | Zendy

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Total-internal-reflection fluorescence microscopy with W-shaped axicon mirrors

Author(s) -

Ming Lei,

Andreas Zumbusch

Publication year - 2010

Publication title -

optics letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.524

H-Index - 272

eISSN - 1071-2763

pISSN - 0146-9592

DOI - 10.1364/ol.35.004057

Subject(s) - total internal reflection fluorescence microscope , optics , microscopy , axicon , fluorescence , light sheet fluorescence microscopy , fluorescence microscope , propidium iodide , materials science , total internal reflection , reflection (computer programming) , laser , chemistry , physics , laser beams , apoptosis , biochemistry , programmed cell death , computer science , programming language

A scheme based on a W-shaped axicon mirror device for total-internal-reflection fluorescence microscopy (TIRFM) is presented. This approach combines the advantages of higher efficiency compared with traditional TIRFM, adjustable illumination area, and simple switching between wide-field and TIRF imaging modes. TIRF images obtained with this approach are free of shadow artifacts and of interference fringes. Example micrographs of fluorescently labeled polystyrene beads, of Convallaria majalis tissue, and of Propidium-iodide-labeled Chinese hamster ovary cells are shown, and the capabilities of the scheme are discussed.

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