Determination of the three-dimensional orientation of single molecules | Zendy

Matthew R. Foreman | Zendy; Carlos Macias-Romero | Zendy; Péter Török | Zendy

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Determination of the three-dimensional orientation of single molecules

Author(s) -

Matthew R. Foreman,

Carlos Macias-Romero,

Péter Török

Publication year - 2008

Publication title -

optics letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.524

H-Index - 272

eISSN - 1071-2763

pISSN - 0146-9592

DOI - 10.1364/ol.33.001020

Subject(s) - optics , electric field , dipole , bandwidth (computing) , orientation (vector space) , physics , component (thermodynamics) , symmetry (geometry) , lens (geology) , cardinal point , computer science , telecommunications , geometry , mathematics , quantum mechanics , thermodynamics

A simple method for real-time determination of the full three-dimensional orientation of the emission dipole of single molecules is presented. Introduction of a pi phase step in the back focal plane of the collector lens breaks the inherent symmetry, allowing the longitudinal dipole component to be measured. Experimental tolerances and the consequence of a nonzero bandwidth emission spectrum are also discussed. The scheme also allows for the longitudinal component of an electric field to be found experimentally.

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