Integrated Raman- and angular-scattering microscopy | Zendy

Zachary J. Smith | Zendy; Andrew J. Berger | Zendy

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Integrated Raman- and angular-scattering microscopy

Author(s) -

Zachary J. Smith,

Andrew J. Berger

Publication year - 2008

Publication title -

optics letters

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.524

H-Index - 272

eISSN - 1071-2763

pISSN - 0146-9592

DOI - 10.1364/ol.33.000714

Subject(s) - optics , raman scattering , mie scattering , microscopy , materials science , scattering , raman spectroscopy , spheres , backscatter (email) , microscope , laser , forward scatter , light scattering , physics , computer science , telecommunications , astronomy , wireless

A microscopy system has been constructed that is capable of simultaneously acquiring both traditional Raman spectra as well as angle-resolved elastic-scattering patterns using a single focused laser spot less than 10 mum wide. The elastic-scattering signal was analyzed by generalized Lorenz-Mie theory, representing what we believe to be the first experimental validation of the theory's prediction of angular backscatter from single spheres. The microscope system exhibits 3 nm precision in predicting sphere diameters, while simultaneously yielding high-quality Raman signals. Applications to single cell analysis are envisioned.

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