Large-image-format computed tomography imaging spectrometer for fluorescence microscopy | Zendy

Bridget K. Ford | Zendy; Michael R. Descour | Zendy; Ronald M. Lynch | Zendy

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Large-image-format computed tomography imaging spectrometer for fluorescence microscopy

Author(s) -

Bridget K. Ford,

Michael R. Descour,

Ronald M. Lynch

Publication year - 2001

Publication title -

optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.394

H-Index - 271

ISSN - 1094-4087

DOI - 10.1364/oe.9.000444

Subject(s) - multispectral image , optics , spectral imaging , microscopy , spectrometer , imaging spectrometer , image resolution , resolution (logic) , fluorescence lifetime imaging microscopy , pixel , materials science , hyperspectral imaging , sampling (signal processing) , point spread function , preclinical imaging , spectral resolution , fluorescence , physics , remote sensing , computer science , in vivo , artificial intelligence , detector , spectral line , geology , microbiology and biotechnology , astronomy , biology

Multispectral imaging has significantly enhanced the analysis of fixed specimens in pathology and cytogenetics. However, application of this technology to in vivo studies has been limited. This is due in part to the increased temporal resolution required to analyze changes in cellular function. Here we present a non-scanning instrument that simultaneously acquires full spectral information (460 nm to 740 nm) from every pixel within its 2-D field of view (200 ìm x 200 ìm) during a single integration time (typically, 2 seconds). The current spatial and spectral sampling intervals of the spectrometer are 0.985 ìm and 5 nm, respectively. These properties allow for the analysis of physiological responses within living biological specimens.

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