Polarization modulation for fluorescence emission difference microscopy | Zendy

Nan Wang | Zendy; Takayoshi Kobayashi | Zendy

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Polarization modulation for fluorescence emission difference microscopy

Author(s) -

Nan Wang,

Takayoshi Kobayashi

Publication year - 2015

Publication title -

optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.394

H-Index - 271

ISSN - 1094-4087

DOI - 10.1364/oe.23.013704

Subject(s) - optics , polarization (electrochemistry) , microscopy , physics , fluorescence , diffraction , excitation , circular polarization , fluorescence microscope , amplitude , subtraction , materials science , chemistry , arithmetic , mathematics , quantum mechanics , microstrip

Effects of the polarization on subtracted images in fluorescence emission difference microscopy are numerically investigated based on vector beam diffraction theory and alteration of Jones matrices. Parameters of resolution, signal loss, and amplitude of negative sidebands after subtraction are discussed along with the polarization of excitation beams and sample sizes. The effects of polarization on the ellipticity and subtraction threshold are also given.

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