A user-friendly two-color super-resolution localization microscope | Zendy

Teng Zhao | Zendy; Ying Wang | Zendy; Yuanliang Zhai | Zendy; Xiaoxuan Qu | Zendy; Aifang Cheng | Zendy; Shengwang Du | Zendy; M. M. T. Loy | Zendy

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A user-friendly two-color super-resolution localization microscope

Author(s) -

Teng Zhao,

Ying Wang,

Yuanliang Zhai,

Xiaoxuan Qu,

Aifang Cheng,

Shengwang Du,

M. M. T. Loy

Publication year - 2015

Publication title -

optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.394

H-Index - 271

ISSN - 1094-4087

DOI - 10.1364/oe.23.001879

Subject(s) - optics , microscopy , microscope , materials science , resolution (logic) , sample (material) , image resolution , computer science , artificial intelligence , chemistry , physics , chromatography

We report a robust two-color method for super-resolution localization microscopy. Two-dye combination of Alexa647 and Alexa750 in an imaging buffer containing COT and using TCEP as switching regent provides matched and balanced switching characteristics for both dyes, allowing simultaneous capture of both on a single camera. Active sample locking stabilizes sample with 1nm accuracy during imaging. With over 4,000 photons emitted from both dyes, two-color superresolution images with high-quality were obtained in a wide range of samples including cell cultures, tissue sections and yeast cells.

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