A novel method of diffraction imaging flow cytometry for sizing microspheres | Zendy

Yu Sa | Zendy; Jun Zhang | Zendy; Marina S. Moran | Zendy; Jun Q. Lu | Zendy; Yuanming Feng | Zendy; XinHua Hu | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

A novel method of diffraction imaging flow cytometry for sizing microspheres

Author(s) -

Yu Sa,

Jun Zhang,

Marina S. Moran,

Jun Q. Lu,

Yuanming Feng,

XinHua Hu

Publication year - 2012

Publication title -

optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.394

H-Index - 271

ISSN - 1094-4087

DOI - 10.1364/oe.20.022245

Subject(s) - optics , diffraction , sizing , materials science , fourier transform , microsphere , light scattering , measure (data warehouse) , image processing , characterization (materials science) , scattering , computer science , physics , image (mathematics) , artificial intelligence , chemistry , organic chemistry , quantum mechanics , database , chemical engineering , engineering

We report a novel method of diffraction imaging flow cytometry to measure and analyze size distribution of microspheres. An automated and robust image processing software based on the short-time-Fourier-transform algorithm has been developed to analyze the characteristic and spatially varying oscillations of side scatters recorded as a diffraction image. Our results demonstrate that the new method allows accurate and rapid determination of single microspheres' diameters ranging from 1 to 100 μm. The capacity for analysis of light scattering by two-sphere aggregates has been demonstrated but analytical tools for characterization of aggregates by multiple microspheres remain to be developed.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research