Adaptive optics enables 3D STED microscopy in aberrating specimens | Zendy

Travis J. Gould | Zendy; Daniel Burke | Zendy; Joerg Bewersdorf | Zendy; Martin J. Booth | Zendy

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Adaptive optics enables 3D STED microscopy in aberrating specimens

Author(s) -

Travis J. Gould,

Daniel Burke,

Joerg Bewersdorf,

Martin J. Booth

Publication year - 2012

Publication title -

optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.394

H-Index - 271

ISSN - 1094-4087

DOI - 10.1364/oe.20.020998

Subject(s) - sted microscopy , optics , microscopy , materials science , adaptive optics , light sheet fluorescence microscopy , interference microscopy , super resolution microscopy , confocal microscopy , scanning confocal electron microscopy , stimulated emission , laser , physics

Stimulated emission depletion (STED) microscopy allows fluorescence far-field imaging with diffraction-unlimited resolution. Unfortunately, extending this technique to three-dimensional (3D) imaging of thick specimens has been inhibited by sample-induced aberrations. Here we present the first implementation of adaptive optics in STED microscopy to allow 3D super-resolution imaging in strongly aberrated imaging conditions, such as those introduced by thick biological tissue.

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