Simultaneous X-ray fluorescence and ptychographic microscopy of Cyclotella meneghiniana | Zendy

D. J. Vine | Zendy; Daniele Pelliccia | Zendy; Christian Holzner | Zendy; Stephen B. Baines | Zendy; Andrew J. Berry | Zendy; Ian McNulty | Zendy; Stefan Vogt | Zendy; Andrew G. Peele | Zendy; K. Nugent | Zendy

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Simultaneous X-ray fluorescence and ptychographic microscopy of Cyclotella meneghiniana

Author(s) -

D. J. Vine,

Daniele Pelliccia,

Christian Holzner,

Stephen B. Baines,

Andrew J. Berry,

Ian McNulty,

Stefan Vogt,

Andrew G. Peele,

K. Nugent

Publication year - 2012

Publication title -

optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.394

H-Index - 271

ISSN - 1094-4087

DOI - 10.1364/oe.20.018287

Subject(s) - optics , microscopy , fluorescence , materials science , resolution (logic) , fluorescence microscope , image resolution , near field scanning optical microscope , optical microscope , physics , scanning electron microscope , artificial intelligence , computer science

Scanning X-ray fluorescence microscopy (XFM) is a particularly useful method for studying the spatial distribution of trace metals in biological samples. Here we demonstrate the utility of combining coherent diffractive imaging (CDI) with XFM for imaging biological samples to simultaneously produce high-resolution and high-contrast transmission images and quantitative elemental maps. The reconstructed transmission function yields morphological details which contextualise the elemental maps. We report enhancement of the spatial resolution in both the transmission and fluorescence images beyond that of the X-ray optics. The freshwater diatom Cyclotella meneghiniana was imaged to demonstrate the benefits of combining these techniques that have complementary contrast mechanisms.

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