Optical refocusing three-dimensional wide-field fluorescence lifetime imaging microscopy | Zendy

Qiang Wu | Zendy; Shangyu Guo | Zendy; Yinxing Ma | Zendy; Feng Gao | Zendy; Chengliang Yang | Zendy; Ming Yang | Zendy; Xuanyi Yu | Zendy; Xinzheng Zhang | Zendy; Romano A. Rupp | Zendy; Jingjun Xu | Zendy

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Optical refocusing three-dimensional wide-field fluorescence lifetime imaging microscopy

Author(s) -

Qiang Wu,

Shangyu Guo,

Yinxing Ma,

Feng Gao,

Chengliang Yang,

Ming Yang,

Xuanyi Yu,

Xinzheng Zhang,

Romano A. Rupp,

Jingjun Xu

Publication year - 2012

Publication title -

optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.394

H-Index - 271

ISSN - 1094-4087

DOI - 10.1364/oe.20.000960

Subject(s) - microscopy , optics , fluorescence , fluorescence microscope , fluorescence lifetime imaging microscopy , materials science , light sheet fluorescence microscopy , fluorescence spectroscopy , optical microscope , photoactivated localization microscopy , fluorescence correlation spectroscopy , fluorescence cross correlation spectroscopy , spectroscopy , physics , multiphoton fluorescence microscope , scanning electron microscope , quantum mechanics

Three-dimensional fluorescence lifetime microscopy is achieved by combining wide-field fluorescence lifetime imaging with a remote optical refocusing method. As required for some applications in dynamic research for physics, chemistry, or biology, it is thereby not necessary to move the sample, i.e., the specimen is not disturbed during measurement. Using a fluorescent microsphere the performance of the system has been tested successfully with respect to three-dimensional fluorescence lifetime microscopy as well as time-resolved fluorescence spectroscopy.

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