Structured light sheet fluorescence microscopy based on four beam interference | Zendy

Ming Lei | Zendy; Andreas Zumbusch | Zendy

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Structured light sheet fluorescence microscopy based on four beam interference

Author(s) -

Ming Lei,

Andreas Zumbusch

Publication year - 2010

Publication title -

optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.394

H-Index - 271

ISSN - 1094-4087

DOI - 10.1364/oe.18.019232

Subject(s) - light sheet fluorescence microscopy , optics , optical sectioning , bright field microscopy , microscopy , materials science , fluorescence microscope , microscope , optical microscope , interference (communication) , beam (structure) , interference microscopy , structured light , pyramid (geometry) , fluorescence , physics , scanning electron microscope , computer science , computer network , channel (broadcasting)

A 3D structured light sheet microscope using a four-faceted symmetric pyramid is presented. The sample is illuminated by the resulting four beam interference field. This approach combines advantages of standing wave and structured illumination microscopy. Examples of micrographs of fluorescently labeled Chinese hamster ovary (CHO) cells as well as of the compound eyes of drosophila are shown and the optical sectioning ability of our system is demonstrated. The capabilities and the limitations of the scheme are discussed.

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