Fast STED microscopy with continuous wave fiber lasers | Zendy

Gaël Moneron | Zendy; Rebecca Medda | Zendy; Birka Hein | Zendy; Arnold Giske | Zendy; Volker Westphal | Zendy; Stefan W. Hell | Zendy

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Fast STED microscopy with continuous wave fiber lasers

Author(s) -

Gaël Moneron,

Rebecca Medda,

Birka Hein,

Arnold Giske,

Volker Westphal,

Stefan W. Hell

Publication year - 2010

Publication title -

optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.394

H-Index - 271

ISSN - 1094-4087

DOI - 10.1364/oe.18.001302

Subject(s) - sted microscopy , optics , microscopy , materials science , stimulated emission , laser , fluorescence , fluorescence microscope , super resolution microscopy , fiber laser , continuous wave , microscope , optoelectronics , physics

We report on fast beam-scanning stimulated-emission-depletion (STED) microscopy in the visible range using for resolution enhancement compact, low cost and turn-key continuous wave (CW) fiber lasers emitting at 592 nm. Spatial resolutions of 35 to 65 nm in the focal plane are shown for various samples including fluorescent nanoparticles, immuno-stained cells with a non-exhaustive selection of 5 commonly used organic fluorescent markers, and living cells expressing the yellow fluorescent protein Citrine. The potential of the straightforward combination of CW-STED and fast beam scanning is illustrated in a movie of the endoplasmic reticulum (ER) of a living cell, composed of 100 frames (6 microm x 12 microm), each of them acquired in a time shorter than 0.2 s.

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