Two-step-only phase-shifting interferometry with optimized detector bandwidth for microscopy of live cells | Zendy

Natan T. Shaked | Zendy; Yizheng Zhu | Zendy; Matthew T. Rinehart | Zendy; Adam Wax | Zendy

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Two-step-only phase-shifting interferometry with optimized detector bandwidth for microscopy of live cells

Author(s) -

Natan T. Shaked,

Yizheng Zhu,

Matthew T. Rinehart,

Adam Wax

Publication year - 2009

Publication title -

optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.394

H-Index - 271

ISSN - 1094-4087

DOI - 10.1364/oe.17.015585

Subject(s) - interferometry , optics , interference microscopy , detector , bandwidth (computing) , microscopy , phase imaging , phase (matter) , materials science , physics , computer science , telecommunications , quantum mechanics

We present a phase-shifting interferometric technique for imaging live biological cells in growth media, while optimizing spatial resolution and enabling potential real-time measurement capabilities. The technique uses slightly-off-axis interferometry which requires less detector bandwidth than traditional off-axis interferometry and fewer measurements than traditional on-axis interferometry. Experimental and theoretical comparisons between the proposed method and these traditional interferometric approaches are given. The method is experimentally demonstrated via phase microscopy of live human skin cancer cells.

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