Two-photon excitation STED microscopy | Zendy

Gaël Moneron | Zendy; Stefan W. Hell | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Two-photon excitation STED microscopy

Author(s) -

Gaël Moneron,

Stefan W. Hell

Publication year - 2009

Publication title -

optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.394

H-Index - 271

ISSN - 1094-4087

DOI - 10.1364/oe.17.014567

Subject(s) - sted microscopy , optics , microscopy , diffraction , laser , fluorescence microscope , materials science , fluorescence , super resolution microscopy , excitation , two photon excitation microscopy , stimulated emission , resolution (logic) , optoelectronics , physics , quantum mechanics , artificial intelligence , computer science

We report sub-diffraction resolution in two-photon excitation (TPE) fluorescence microscopy achieved by merging this technique with stimulated-emission depletion (STED). We demonstrate an easy-to-implement and promising laser combination based on a short-pulse laser source for two-photon excitation and a continuous-wave (CW) laser source for resolution enhancement. Images of fluorescent nanoparticles and the immunostained transcription regulator NF kappaB in mammalian cell nuclei exhibit resolutions of <50 nm and approximately 70 nm in the focal plane, respectively, corresponding to a 4-5.4-fold improvement over the diffraction barrier.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research