Estimation of the effective orientation of the SHG source in primary cortical neurons | Zendy

Sotiris Psilodimitrakopoulos | Zendy; Valérie Petegnief | Zendy; Guadalupe Sòria | Zendy; Ivan AmatRoldan | Zendy; David Artigas | Zendy; Anna M. Planas | Zendy; Pablo LozaÁlvarez | Zendy

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Estimation of the effective orientation of the SHG source in primary cortical neurons

Author(s) -

Sotiris Psilodimitrakopoulos,

Valérie Petegnief,

Guadalupe Sòria,

Ivan AmatRoldan,

David Artigas,

Anna M. Planas,

Pablo LozaÁlvarez

Publication year - 2009

Publication title -

optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.394

H-Index - 271

ISSN - 1094-4087

DOI - 10.1364/oe.17.014418

Subject(s) - optics , second harmonic generation , microscopy , second harmonic imaging microscopy , orientation (vector space) , polarization (electrochemistry) , dipole , materials science , pixel , bandwidth (computing) , physics , laser , computer science , chemistry , telecommunications , geometry , mathematics , quantum mechanics

In this paper we provide, for the first time to our knowledge, the effective orientation of the SHG source in cultured cortical neuronal processes in vitro. This is done by the use of the polarization sensitive second harmonic generation (PSHG) imaging microscopy technique. By performing a pixel-level resolution analysis we found that the SHG dipole source has a distribution of angles centered at thetae =33.96 degrees , with a bandwidth of Deltathetae = 12.85 degrees . This orientation can be related with the molecular geometry of the tubulin heterodimmer contained in microtubules.

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