Annular pupil filter under shot-noise condition for linear and non linear microscopy | Zendy

Emiliano Ronzitti | Zendy; Giuseppe Vicidomini | Zendy; Valentina Caorsi | Zendy; Alberto Diaspro | Zendy

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Annular pupil filter under shot-noise condition for linear and non linear microscopy

Author(s) -

Emiliano Ronzitti,

Giuseppe Vicidomini,

Valentina Caorsi,

Alberto Diaspro

Publication year - 2009

Publication title -

optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.394

H-Index - 271

ISSN - 1094-4087

DOI - 10.1364/oe.17.006867

Subject(s) - optics , microscope , microscopy , shot noise , materials science , lens (geology) , spatial filter , filter (signal processing) , light sheet fluorescence microscopy , noise (video) , optical sectioning , physics , linear filter , detector , scanning confocal electron microscopy , computer science , artificial intelligence , image (mathematics) , computer vision

The imaging performances of multiphoton excitation and confocal laser scanning microscopy are herby considered: in typical experimental imaging conditions, a small finite amount of photon reaches the detector giving shot-noise fluctuations which affects the signal acquired. A significant detriment in the high frequencies transmission capability is obtained. In order to partially recover the high frequencies information lost, the insertion of a pupil plane filter in the microscope illumination light pathway on the objective lens is proposed. We demonstrate high-frequency and resolution enhancement in the case of linear and non linear fluorescence microscope approach under shot-noise condition.

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