Interference terahertz label-free imaging for protein detection on a membrane | Zendy

Yuichi Ogawa | Zendy; Shin’ichiro Hayashi | Zendy; Masato Oikawa | Zendy; Chiko Otani | Zendy; Kodo Kawase | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

Interference terahertz label-free imaging for protein detection on a membrane

Author(s) -

Yuichi Ogawa,

Shin’ichiro Hayashi,

Masato Oikawa,

Chiko Otani,

Kodo Kawase

Publication year - 2008

Publication title -

optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.394

H-Index - 271

ISSN - 1094-4087

DOI - 10.1364/oe.16.022083

Subject(s) - terahertz radiation , terahertz spectroscopy and technology , materials science , ethylene glycol , terahertz time domain spectroscopy , streptavidin , membrane , spectroscopy , optics , optoelectronics , chemistry , biotin , physics , biochemistry , organic chemistry , quantum mechanics

We demonstrate a highly sensitive imaging method combined a terahertz time-domain spectroscopy and an interference effect for label-free protein detection on a polyvinylidene difluoride membrane. The method is based on terahertz time-domain spectroscopy and uses an interference effect. Biotin is linked to the membrane using poly ethylene glycol or poly ethylene glycol methyl ether to prevent it from being washed off. Binding of the biotin with streptavidin is then observed by measuring the terahertz signal change due to the variation of the membrane refractive index. We demonstrate the detection of the binding streptavidin protein in gradually decreasing concentrations, down to 27 ng mm-2, using the image recorded at 1.5 THz.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research