Detection of fluorescent-labeled probes at sub-picomolar concentrations by magnetic modulation | Zendy

Amos Danielli | Zendy; Ady Arie | Zendy; Noga Porat | Zendy; Marcelo Ehrlich | Zendy

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Detection of fluorescent-labeled probes at sub-picomolar concentrations by magnetic modulation

Author(s) -

Amos Danielli,

Ady Arie,

Noga Porat,

Marcelo Ehrlich

Publication year - 2008

Publication title -

optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.394

H-Index - 271

ISSN - 1094-4087

DOI - 10.1364/oe.16.019253

Subject(s) - fluorescence , optics , materials science , magnetic field , signal (programming language) , scattering , modulation (music) , nuclear magnetic resonance , light scattering , noise (video) , analytical chemistry (journal) , chemistry , physics , chromatography , quantum mechanics , artificial intelligence , computer science , acoustics , image (mathematics) , programming language

A sensitive and rapid method for detecting fluorescent dyes at low concentrations in homogenous solution is experimentally demonstrated. Fluorescent-labeled DNA probes are detected by attaching magnetic beads and applying alternating magnetic field gradient. This condenses the fluorescent probes into a small detection volume and eliminates the scattering noise from solution by synchronous detection. For DNA probes concentration of 1 x 10(-13) M the detection signal was 3.3 times higher than the noise, thereby implying detection sensitivity of 3 x 10(-14) M.

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