Advancing multifocal nonlinear microscopy: development and application of a novel multibeam Yb:KGd(WO_4)_2 oscillator
Author(s) -
Kraig E. Sheetz,
Erich E. Hoover,
Ramn Carriles,
David Kleinfeld,
Jeff Squier
Publication year - 2008
Publication title -
optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.394
H-Index - 271
ISSN - 1094-4087
DOI - 10.1364/oe.16.017574
Subject(s) - optics , microscopy , multiplexer , second harmonic generation , nonlinear optics , materials science , high harmonic generation , nonlinear system , physics , laser , multiplexing , computer science , telecommunications , quantum mechanics
We present a novel Yb:KGd(WO(4))(2) oscillator design that generates six beams of temporally delayed, 253 fs, 11 nJ pulses. This allows multifocal nonlinear microscopy to be performed without the need for complicated optical multiplexers. We demonstrate our design with twelve simultaneously acquired two-photon, second-harmonic and/or third-harmonic images generated from six laterally separated foci.
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