Nonlinear immunofluorescent assay for androgenic hormones based on resonant structures | Zendy

Anisha Thayil | Zendy; Alejandro Muriano | Zendy; J.Pablo Salvador | Zendy; Roger Galvé | Zendy; M.Pilar Marco | Zendy; Dobryna Zalvidea | Zendy; Pablo LozaÁlvarez | Zendy; Tsvi Katchalski | Zendy; Eran Grinvald | Zendy; Asher A. Friesem | Zendy; Silvia Soria | Zendy

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Nonlinear immunofluorescent assay for androgenic hormones based on resonant structures

Author(s) -

Anisha Thayil,

Alejandro Muriano,

J.Pablo Salvador,

Roger Galvé,

M.Pilar Marco,

Dobryna Zalvidea,

Pablo LozaÁlvarez,

Tsvi Katchalski,

Eran Grinvald,

Asher A. Friesem,

Silvia Soria

Publication year - 2008

Publication title -

optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.394

H-Index - 271

ISSN - 1094-4087

DOI - 10.1364/oe.16.013315

Subject(s) - fluorescence , optics , grating , nonlinear optics , anabolism , chemistry , biophysics , materials science , physics , biology , biochemistry , laser

We report for the first time the use of two photon fluorescence as detection method of affinity binding reactions. We use a resonant grating waveguide structure as platform enhancement for detecting the interaction between fluorescent labeled Boldenone, a non-natural androgenic hormone, and a specific anti-anabolic antibody. We were able to detect a surface coverage of approximately 0.7 ng/mm(2).

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