4Pi microscopy of type A with 1-photon excitation in biological fluorescence imaging | Zendy

Marion Lang | Zendy; Tobias Müller | Zendy; Johann Engelhardt | Zendy; Stefan W. Hell | Zendy

AI Assistant Blog Pricing

Home ZAIA Blog

Open Access

4Pi microscopy of type A with 1-photon excitation in biological fluorescence imaging

Author(s) -

Marion Lang,

Tobias Müller,

Johann Engelhardt,

Stefan W. Hell

Publication year - 2007

Publication title -

optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.394

H-Index - 271

ISSN - 1094-4087

DOI - 10.1364/oe.15.002459

Subject(s) - optics , microscopy , deconvolution , materials science , two photon excitation microscopy , light sheet fluorescence microscopy , fluorescence lifetime imaging microscopy , resolution (logic) , fluorescence microscope , microscope , fluorescence , confocal microscopy , image resolution , scanning confocal electron microscopy , physics , artificial intelligence , computer science

We demonstrate that oil immersion lenses with a semi-aperture angle >/= 74 degrees enable 4Pi confocal fluorescence microscopy of type A with 1 photon excitation. The axial sidelobes amount to < 50 % of the main diffraction maximum, implying that lobe induced artifacts can be removed from the image data. The advancement reported herein enables a relative inexpensive implementation of 4Pi microscopy, providing axially superresolved 3D-imaging in transparent samples. As an example, we show dual-color 4Pi images of double stained Golgi stacks in a mammalian cell with 110 nm axial resolution. The resolution can be further enhanced to values slightly below 100 nm by image deconvolution.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.

Having issues? You can contact us here

Accelerating Research