Enhanced Green Fluorescent Protein (GFP) fluorescence after polyelectrolyte caging | Zendy

Alberto Diaspro | Zendy; Silke Krol | Zendy; Barbara Campanini | Zendy; Fabio Can | Zendy; Giuseppe Chirico | Zendy

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Enhanced Green Fluorescent Protein (GFP) fluorescence after polyelectrolyte caging

Author(s) -

Alberto Diaspro,

Silke Krol,

Barbara Campanini,

Fabio Can,

Giuseppe Chirico

Publication year - 2006

Publication title -

optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.394

H-Index - 271

ISSN - 1094-4087

DOI - 10.1364/oe.14.009815

Subject(s) - green fluorescent protein , fluorescence , polyelectrolyte , materials science , fluorescence spectroscopy , biophysics , fluorescence correlation spectroscopy , fluorescent protein , fluorescence in the life sciences , molecule , molecular biophysics , nanotechnology , spectroscopy , optics , chemistry , polymer , biology , physics , biochemistry , organic chemistry , quantum mechanics , composite material , gene

Discovery of Green Fluorescent Protein (GFP) constituted an important improvement for living cell studies on submicron resolution allowing in vivo fluorescence labeling. We studied the photo-physical properties of single GFP molecules incorporated in a charged polyelectrolyte environment by means of single molecule spectroscopy. The fluorescence characteristics change dramatically in terms of photo-stability,lifetime and blinking behavior so that the proteins scale up to quantum dots. The reported results highlight interesting applications in the design of fluorescent markers and in the development of optical data storage architectures.

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