The application of frequency-domain Fluorescence Lifetime Imaging Microscopy as a quantitative analytical tool for microfluidic devices | Zendy

Alan D. Elder | Zendy; Sinéad M. Matthews | Zendy; Johannes Swartling | Zendy; Kamran Yunus | Zendy; Jonathan H. Frank | Zendy; Conor Brennan | Zendy; Adrian C. Fisher | Zendy; Clemens F. Kaminski | Zendy

Open Access

The application of frequency-domain Fluorescence Lifetime Imaging Microscopy as a quantitative analytical tool for microfluidic devices

Author(s) -

Alan D. Elder,

Sinéad M. Matthews,

Johannes Swartling,

Kamran Yunus,

Jonathan H. Frank,

Conor Brennan,

Adrian C. Fisher,

Clemens F. Kaminski

Publication year - 2006

Publication title -

optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.394

H-Index - 271

ISSN - 1094-4087

DOI - 10.1364/oe.14.005456

Subject(s) - fluorescence lifetime imaging microscopy , microfluidics , materials science , microscopy , optics , microchannel , fluorescence , optoelectronics , live cell imaging , fluorescence microscope , nanotechnology , chemistry , physics , biochemistry , cell

We describe the application of wide-field frequency domain Fluorescence Lifetime Imaging Microscopy (FLIM) to imaging in microfluidic devices. FLIM is performed using low cost, intensity modulated Light Emitting Diodes (LEDs) for illumination. The use of lifetime imaging for quantitative analysis within such devices is demonstrated by mapping the molecular diffusion of iodide ions across a microchannel.

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