Non-mechanically-axial-scanning confocal microscope using adaptive mirror switching | Zendy

Yoshiaki Yasuno | Zendy; Shuichi Makita | Zendy; Toyohiko Yatagai | Zendy; Tobias Wiesendanger | Zendy; Aiko Ruprecht | Zendy; Hans J. Tiziani | Zendy

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Non-mechanically-axial-scanning confocal microscope using adaptive mirror switching

Author(s) -

Yoshiaki Yasuno,

Shuichi Makita,

Toyohiko Yatagai,

Tobias Wiesendanger,

Aiko Ruprecht,

Hans J. Tiziani

Publication year - 2003

Publication title -

optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.394

H-Index - 271

ISSN - 1094-4087

DOI - 10.1364/oe.11.000054

Subject(s) - confocal , optics , monochromatic color , microscope , confocal microscopy , materials science , point spread function , adaptive optics , physics

A non-axial-scanning confocal microscope employing a monochromatic light source has been developed. The system controls the defocus of an objective into three to .ve optimized states by using a membrane-adaptive mirror, and determines the axial height of an object according to the confocal output value with each defocus. A genetic algorithm is employed to optimize the adaptive mirror shape, with the information entropy of the spectrum of the lateral confocal spot pro.le used as a cost function in the genetic algorithm. Our experimental system successfully determined axial object height within 50 microm range with 0.64 % of error.

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