Rapid, large-scale stimulated Raman histology with strip mosaicing and dual-phase detection | Zendy

Bohan Zhang | Zendy; Mengxiong Sun | Zendy; Yifan Yang | Zendy; Lingchao Chen | Zendy; Xiang Zou | Zendy; Tian Yang | Zendy; Yingqi Hua | Zendy; Minbiao Ji | Zendy

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Rapid, large-scale stimulated Raman histology with strip mosaicing and dual-phase detection

Author(s) -

Bohan Zhang,

Mengxiong Sun,

Yifan Yang,

Lingchao Chen,

Xiang Zou,

Tian Yang,

Yingqi Hua,

Minbiao Ji

Publication year - 2018

Publication title -

biomedical optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.362

H-Index - 86

ISSN - 2156-7085

DOI - 10.1364/boe.9.002604

Subject(s) - raman spectroscopy , optics , phase (matter) , phase imaging , materials science , computer science , artificial intelligence , microscopy , physics , quantum mechanics

Two-color stimulated Raman scattering (SRS) microscopy with label-free mapping of lipid/protein distributions has shown promise in virtual histology. Despite previous demonstrations of SRS in tumor delineation and diagnosis, the speed and efficiency of the current technique requires further improvements for practical use. Here, we integrate parallel dual-phase SRS detection with strip mosaicing, which reduces the imaging time of a whole mouse brain section from 70 to 8 minutes. We further verified our method in imaging fresh human surgical tissues, showing its great potential for rapid SRS histology, especially for large scale, large quantity imaging tasks.

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