Multifocus structured illumination microscopy for fast volumetric super-resolution imaging | Zendy

Sara Abrahamsson | Zendy; Hans Blom | Zendy; Ana Agostinho | Zendy; Daniel C. Jans | Zendy; A Jost | Zendy; Marcel Müller | Zendy; Linnéa Nilsson | Zendy; Kristoffer Bernhem | Zendy; Talley J. Lambert | Zendy; Rainer Heintzmann | Zendy; Hjalmar Brismar | Zendy

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Multifocus structured illumination microscopy for fast volumetric super-resolution imaging

Author(s) -

Sara Abrahamsson,

Hans Blom,

Ana Agostinho,

Daniel C. Jans,

A Jost,

Marcel Müller,

Linnéa Nilsson,

Kristoffer Bernhem,

Talley J. Lambert,

Rainer Heintzmann,

Hjalmar Brismar

Publication year - 2017

Publication title -

biomedical optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.362

H-Index - 86

ISSN - 2156-7085

DOI - 10.1364/boe.8.004135

Subject(s) - microscopy , optics , biological imaging , image resolution , resolution (logic) , light sheet fluorescence microscopy , superresolution , diffraction , medical imaging , materials science , computer science , computer vision , artificial intelligence , physics , scanning confocal electron microscopy , image (mathematics) , fluorescence

We here report for the first time the synergistic implementation of structured illumination microscopy (SIM) and multifocus microscopy (MFM). This imaging modality is designed to alleviate the problem of insufficient volumetric acquisition speed in super-resolution biological imaging. SIM is a wide-field super-resolution technique that allows imaging with visible light beyond the classical diffraction limit. Employing multifocus diffractive optics we obtain simultaneous wide-field 3D imaging capability in the SIM acquisition sequence, improving volumetric acquisition speed by an order of magnitude. Imaging performance is demonstrated on biological specimens.

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