Open AccessFast 3D visualization of endogenous brain signals with high-sensitivity laser scanning photothermal microscopy
Author(s) -
Jun Miyazaki,
Tadatsune Iida,
Shinji Tanaka,
Akiko HayashiTakagi,
Haruo Kasai,
Shigeo Okabe,
Takayoshi Kobayashi
Publication year - 2016
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.7.001702
Subject(s) - photothermal therapy , materials science , microscope , microscopy , confocal , laser , confocal microscopy , optics , fluorescence , fluorescence microscope , fluorescence lifetime imaging microscopy , signal (programming language) , photothermal effect , biomedical engineering , nanotechnology , computer science , medicine , physics , programming language
A fast, high-sensitivity photothermal microscope was developed by implementing a spatially segmented balanced detection scheme into a laser scanning microscope. We confirmed a 4.9 times improvement in signal-to-noise ratio in the spatially segmented balanced detection compared with that of conventional detection. The system demonstrated simultaneous bi-modal photothermal and confocal fluorescence imaging of transgenic mouse brain tissue with a pixel dwell time of 20 μs. The fluorescence image visualized neurons expressing yellow fluorescence proteins, while the photothermal signal detected endogenous chromophores in the mouse brain, allowing 3D visualization of the distribution of various features such as blood cells and fine structures probably due to lipids. This imaging modality was constructed using compact and cost-effective laser diodes, and will thus be widely useful in the life and medical sciences.
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