U-shaped, double-tapered, fiber-optic sensor for effective biofilm growth monitoring
Author(s) -
Nianbing Zhong,
Mingfu Zhao,
Yishan Li
Publication year - 2016
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.7.000335
Subject(s) - biofilm , polydimethylsiloxane , photobioreactor , materials science , absorption (acoustics) , optical fiber , fiber , adsorption , optics , polyimide , nanotechnology , layer (electronics) , biomass (ecology) , composite material , chemistry , oceanography , genetics , physics , organic chemistry , geology , bacteria , biology
To monitor biofilm growth on polydimethylsiloxane in a photobioreactor effectively, the biofilm cells and liquids were separated and measured using a sensor with two U-shaped, double-tapered, fiber-optic probes (Sen. and Ref. probes). The probes' Au-coated hemispherical tips enabled double-pass evanescent field absorption. The Sen. probe sensed the cells and liquids inside the biofilm. The polyimide-silica hybrid-film-coated Ref. probe separated the liquids from the biofilm cells and analyzed the liquid concentration. The biofilm structure and active biomass were also examined to confirm the effectiveness of the measurement using a simulation model. The sensor was found to effectively respond to the biofilm growth in the adsorption through exponential phases at thicknesses of 0-536 μm.
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