Open AccessEffect of recombinant interleukin-12 on murine skin regeneration and cell dynamics using in vivo multimodal microscopy
Author(s) -
Joanne Li,
Andrew J. Bower,
Vladimir Vainstein,
Zoya GluzmanPoltorak,
Eric J. Chaney,
Marina Marjanović,
Lena A Basile,
Stephen A. Boppart
Publication year - 2015
Publication title -
biomedical optics express
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.362
H-Index - 86
ISSN - 2156-7085
DOI - 10.1364/boe.6.004277
Subject(s) - in vivo , microscopy , recombinant dna , microbiology and biotechnology , dynamics (music) , multiphoton fluorescence microscope , regeneration (biology) , interleukin , pathology , biology , immunology , cytokine , medicine , optics , fluorescence microscope , physics , biochemistry , fluorescence , gene , acoustics
Interleukin-12 (IL-12) is a pro-inflammatory cytokine known for its role in immunity, and previous studies have shown that IL-12 provides mitigation of radiation injury. In this study, we utilize a multimodal microscopy system equipped with second harmonic generation (SHG) and fluorescence lifetime imaging microscopy (FLIM) to examine the effect of IL-12 on collagen structure and cellular metabolic activity in vivo during skin wound healing. This preliminary study illustrates the highly dynamic and heterogeneous in vivo microenvironment of the wounded skin. In addition, results suggest that IL-12 triggers a significantly more rapid and greater cellular metabolic response in the wounded animals. These results can elucidate insights into the response mechanism of IL-12 in both wound healing and acute radiation syndrome.
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