Screening hypochromism (sieve effect) in red blood cells: a quantitative analysis | Zendy

K. Razi Naqvi | Zendy

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Screening hypochromism (sieve effect) in red blood cells: a quantitative analysis

Author(s) -

K. Razi Naqvi

Publication year - 2014

Publication title -

biomedical optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.362

H-Index - 86

ISSN - 2156-7085

DOI - 10.1364/boe.5.001290

Subject(s) - suspension (topology) , absorption (acoustics) , spectroscopy , chemistry , scattering , red shift , absorption spectroscopy , light scattering , quantitative analysis (chemistry) , spectral line , optics , materials science , analytical chemistry (journal) , physics , chromatography , mathematics , quantum mechanics , astronomy , homotopy , galaxy , pure mathematics

Multiwavelength UV-visible spectroscopy, Kramers-Kronig analysis, and several other experimental and theoretical tools have been applied over the last several decades to fathom absorption and scattering of light by suspensions of micron-sized pigmented particles, including red blood cells, but a satisfactory quantitative analysis of the difference between the absorption spectra of suspension of intact and lysed red blood cells is still lacking. It is stressed that such a comparison is meaningful only if the pertinent spectra are free from, or have been corrected for, scattering losses, and it is shown that Duysens' theory can, whereas that of Vekshin cannot, account satisfactorily for the observed hypochromism of suspensions of red blood cells.

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