Effect of temperature and fixation on the optical properties of atherosclerotic tissue: a validation study of an ex-vivo whole heart cadaveric model | Zendy

Muthukaruppan Gnanadesigan | Zendy; Gijs van Soest | Zendy; Stephen J. White | Zendy; Simon Scoltock | Zendy; Giovanni Jacopo Ughi | Zendy; Andreas Baumbach | Zendy; Antonius F. van der Steen | Zendy; Evelyn Regar | Zendy; Tom Johnson | Zendy

Open Access

Effect of temperature and fixation on the optical properties of atherosclerotic tissue: a validation study of an ex-vivo whole heart cadaveric model

Author(s) -

Muthukaruppan Gnanadesigan,

Gijs van Soest,

Stephen J. White,

Simon Scoltock,

Giovanni Jacopo Ughi,

Andreas Baumbach,

Antonius F. van der Steen,

Evelyn Regar,

Tom Johnson

Publication year - 2014

Publication title -

biomedical optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.362

H-Index - 86

ISSN - 2156-7085

DOI - 10.1364/boe.5.001038

Subject(s) - optical coherence tomography , ex vivo , attenuation coefficient , attenuation , cadaveric spasm , biomedical engineering , fixation (population genetics) , materials science , optics , in vivo , pathology , medicine , anatomy , physics , biology , population , microbiology and biotechnology , environmental health

Atherosclerotic plaque composition can be imaged using the optical attenuation coefficient derived from intravascular optical coherence tomography (OCT) data. The relation between optical properties and tissue type has been established on autopsy tissues. In this study, we validate an ex-vivo model for the effect of temperature and tissue fixation on optical parameters. We studied the optical attenuation of human coronary arteries at three temperatures, before and after formalin fixation. We developed an en-face longitudinal display of attenuation data of the OCT pullbacks. Using the unfixed, body-temperature condition image as a standard, and after extensive registration with other condition images, we quantify the differences in optical attenuation and the backscattered intensity. The results suggest that tissue fixation and temperature do not introduce systematic errors in studies of arterial optical properties.

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