Fluorescence lifetime spectroscopy of tissue autofluorescence in normal and diseased colon measured ex vivo using a fiber-optic probe | Zendy

Sergio Coda | Zendy; Alex J. Thompson | Zendy; Gordon T. Kennedy | Zendy; Kim L. Roche | Zendy; Lakshmana Ayaru | Zendy; Devinder S. Bansi | Zendy; Gordon Stamp | Zendy; Andrew V. Thillainayagam | Zendy; P. M. W. French | Zendy; Chris Dunsby | Zendy

Open Access

Fluorescence lifetime spectroscopy of tissue autofluorescence in normal and diseased colon measured ex vivo using a fiber-optic probe

Author(s) -

Sergio Coda,

Alex J. Thompson,

Gordon T. Kennedy,

Kim L. Roche,

Lakshmana Ayaru,

Devinder S. Bansi,

Gordon Stamp,

Andrew V. Thillainayagam,

P. M. W. French,

Chris Dunsby

Publication year - 2014

Publication title -

biomedical optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.362

H-Index - 86

ISSN - 2156-7085

DOI - 10.1364/boe.5.000515

Subject(s) - autofluorescence , ex vivo , pathology , materials science , fluorescence , biopsy , fluorescence spectroscopy , optics , in vivo , medicine , biology , physics , microbiology and biotechnology

We present an ex vivo study of temporally and spectrally resolved autofluorescence in a total of 47 endoscopic excision biopsy/resection specimens from colon, using pulsed excitation laser sources operating at wavelengths of 375 nm and 435 nm. A paired analysis of normal and neoplastic (adenomatous polyp) tissue specimens obtained from the same patient yielded a significant difference in the mean spectrally averaged autofluorescence lifetime -570 ± 740 ps (p = 0.021, n = 12). We also investigated the fluorescence signature of non-neoplastic polyps (n = 6) and inflammatory bowel disease (n = 4) compared to normal tissue in a small number of specimens.

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