Erythrocyte volumetric measurements in imaging flow cytometry using simultaneous three-wavelength digital holographic microscopy | Zendy

Nir A. Turko | Zendy; Natan T. Shaked | Zendy

Open Access

Erythrocyte volumetric measurements in imaging flow cytometry using simultaneous three-wavelength digital holographic microscopy

Author(s) -

Nir A. Turko,

Natan T. Shaked

Publication year - 2020

Publication title -

biomedical optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.362

H-Index - 86

ISSN - 2156-7085

DOI - 10.1364/boe.404368

Subject(s) - digital holographic microscopy , sphericity , materials science , microscopy , refractive index , mean corpuscular volume , wavelength , microfluidics , phase imaging , optics , holography , digital holography , absorption (acoustics) , phase (matter) , chemistry , hemoglobin , nanotechnology , optoelectronics , physics , biochemistry , organic chemistry , composite material

We report a cross-talk free simultaneous three-wavelength digital holographic microscopy setup for spectroscopic imaging of biological cells during flow. The feasibility of the proposed measurement technique is demonstrated on erythrocytes, due to their unique morphology and dependency of hemoglobin (Hb) molecule absorption on wavelength. From the spectroscopic quantitative phase profiles of cells acquired during flow in a microfluidic device, we decoupled the refractive index and the physical thickness. We then used our quantitative phase imaging results to dynamically calculate the mean cell volume (MCV), mean corpuscular Hb concentration (MCHC), mean corpuscular Hb content (MCH) and sphericity index.

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