Quantitative laser speckle flowmetry of the in vivo microcirculation using sidestream dark field microscopy | Zendy

Annemarie Nadort | Zendy; Rutger G. Woolthuis | Zendy; Ton G. van Leeuwen | Zendy; Dirk J. Faber | Zendy

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Quantitative laser speckle flowmetry of the in vivo microcirculation using sidestream dark field microscopy

Author(s) -

Annemarie Nadort,

Rutger G. Woolthuis,

Ton G. van Leeuwen,

Dirk J. Faber

Publication year - 2013

Publication title -

biomedical optics express

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.362

H-Index - 86

ISSN - 2156-7085

DOI - 10.1364/boe.4.002347

Subject(s) - speckle pattern , decorrelation , microcirculation , optics , speckle imaging , biomedical engineering , materials science , physics , medicine , computer science , computer vision , radiology

We present integrated Laser Speckle Contrast Imaging (LSCI) and Sidestream Dark Field (SDF) flowmetry to provide real-time, non-invasive and quantitative measurements of speckle decorrelation times related to microcirculatory flow. Using a multi exposure acquisition scheme, precise speckle decorrelation times were obtained. Applying SDF-LSCI in vitro and in vivo allows direct comparison between speckle contrast decorrelation and flow velocities, while imaging the phantom and microcirculation architecture. This resulted in a novel analysis approach that distinguishes decorrelation due to flow from other additive decorrelation sources.

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